NOS1 antibody (pSer852)

Catalog No. ABIN6256437

The Rabbit Polyclonal anti-NOS1 antibody is suitable to detect NOS1 in samples from Human, Rat and Mouse. It has been validated for WB, IHC, ELISA, ICC and IF.

Quick Overview for NOS1 antibody (pSer852) (ABIN6256437)

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-NOS1 Antibody

Binding Specificity: pSer852

Specificity: Phospho-nNOS (Ser852) Antibody detects endogenous levels of nNOS only when phosphorylated at Serine 852.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Rabbit,Dog,Chicken,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human nNOS around the phosphorylation site of Ser852.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Alternative Name: NOS1

Background:

Description: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

Gene: NOS1

Molecular Weight: 120/160kDa

Gene ID: 4842

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction