CSNK1A1 antibody (pTyr294)

Catalog No. ABIN6256701

The Rabbit Polyclonal anti-CSNK1A1 antibody is suitable to detect CSNK1A1 in samples from Human, Rat and Mouse. It has been validated for WB, IHC, ELISA, IF and ICC.

Quick Overview for CSNK1A1 antibody (pTyr294) (ABIN6256701)

Target: CSNK1A1 (Casein Kinase 1, alpha 1 (CSNK1A1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CSNK1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CSNK1A1 Antibody

Binding Specificity: pTyr294

Specificity: Phospho-CK-1 alpha (Tyr294) Antibody detects endogenous levels of CK-1 alpha only when phosphorylated at PhoTyr294

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Phospho-CK-1 alpha (Tyr294)

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000 IHC 1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for CSNK1A1

Target: CSNK1A1 (Casein Kinase 1, alpha 1 (CSNK1A1))

Alternative Name: CK-1 alpha

Background:

Description: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis (PubMed:11955436, PubMed:1409656, PubMed:18305108). May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration (PubMed:23902688).

Gene: CSNK1A1

Molecular Weight: 38 kDa

Gene ID: 1452

UniProt: P48729, Q8N752

Pathways: WNT Signaling, Hedgehog Signaling