PIK3R1 antibody (pTyr607)

Catalog No. ABIN6256775

This Rabbit Polyclonal antibody specifically detects PIK3R1 in WB, ELISA, IHC, IF and ICC. It exhibits reactivity toward Human, Mouse, Rat and Pig and has been mentioned in 5+ publications.

Quick Overview for PIK3R1 antibody (pTyr607) (ABIN6256775)

Target: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3R1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PIK3R1 Antibody

Binding Specificity: pTyr607

Specificity: Phospho-PI3-kinase p85- alpha (Tyr607) Antibody detects endogenous levels of PI3-kinase p85- alpha only when phosphorylated at Tyrosine 607

Cross-Reactivity: Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human PI3-kinase p85- alpha around the phosphorylation site of Tyrosine 607

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

References for anti-PIK3R1 antibody (ABIN6256775)

Chang, Guo, Zhou, Huang, Yi, Dou, Huan: "HBP induces the expression of monocyte chemoattractant protein-1 via the FAK/PI3K/AKT and p38 MAPK/NF-κB pathways in vascular endothelial cells." in: Cellular signalling, Vol. 43, pp. 85-94, (2018) (PubMed).

Chai, Bai, Li, Chen, Zhang: "Biological functions of lung cancer cells are suppressed in co-culture with mesenchymal stem cells isolated from umbilical cord." in: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 1076-1080, (2018) (PubMed).

Liu, Geng, Zhang, Wang, Zhang, Duan, Zhang: "Oligo-Porphyran Ameliorates Neurobehavioral Deficits in Parkinsonian Mice by Regulating the PI3K/Akt/Bcl-2 Pathway." in: Marine drugs, Vol. 16, Issue 3, (2018) (PubMed).

Wang, Li, Chen, Yang: "Umbilical cord‑derived mesenchymal stem cells can inhibit the biological functions of melanoma A375 cells." in: Oncology reports, Vol. 40, Issue 1, pp. 511-517, (2018) (PubMed).

Shang, Wang, Tong, Kang, Liang, Ge: "Prolyl hydroxylases positively regulated LPS-induced inflammation in human gingival fibroblasts via TLR4/MyD88-mediated AKT/NF-κB and MAPK pathways." in: Cell proliferation, pp. e12516, (2018) (PubMed).

Target Details for PIK3R1

Target: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Alternative Name: PI3K p85 alpha

Background:

Description: Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).

Gene: PIK3R1

Molecular Weight: 80kDa

Gene ID: 5295

UniProt: P27986

Pathways: TCR Signaling, Response to Growth Hormone Stimulus, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Hepatitis C, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling, Warburg Effect