EPS15 antibody (pTyr849)

Catalog No. ABIN6256811

This Rabbit Polyclonal antibody specifically detects EPS15 in WB, ELISA, IHC, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for EPS15 antibody (pTyr849) (ABIN6256811)

Target: EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPS15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-EPS15 Antibody

Binding Specificity: pTyr849

Specificity: Phospho-EPS15 (Tyr849) Antibody detects endogenous levels of EPS15 only when phosphorylated at Tyrosine 849.

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human EPS15 around the phosphorylation site of Tyr849.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for EPS15

Target: EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15))

Alternative Name: EPS15

Background:

Description: Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR), internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.

Gene: EPS15

Molecular Weight: 98kDa

Gene ID: 2060

UniProt: P42566

Pathways: EGFR Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, EGFR Downregulation