EPS15 antibody (pTyr849)

Catalog No. ABIN7220316

The Rabbit Polyclonal anti-EPS15 antibody (ABIN7220316) specifically detects EPS15 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for EPS15 antibody (pTyr849) (ABIN7220316)

Target: EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPS15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-EPS15 Antibody

Binding Specificity: pTyr849

Purpose: Rabbit Anti-Eps15 (phospho Tyr849) Polyclonal Antibody

Specificity: Phospho-Eps15 (Y849) Polyclonal Antibody detects endogenous levels of Eps15 protein only when phosphorylated at Y849.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Eps15 Phospho-Tyr849

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EPS15

Target: EPS15 (Epidermal Growth Factor Receptor Pathway Substrate 15 (EPS15))

Alternative Name: Eps15

Background: EPS15, AF1P, Epidermal growth factor receptor substrate 15, Protein Eps15, Protein AF-1pEPS15 encodes epidermal growth factor receptor pathway substrate 15 that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, EPS15 is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Molecular Weight: 100kD

Gene ID: 2060

UniProt: P42566

Pathways: EGFR Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, EGFR Downregulation