ATF3 antibody (C-Term)

Catalog No. ABIN6256914

The Rabbit Polyclonal anti-ATF3 antibody is suitable to detect ATF3 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, ICC and IF.

Quick Overview for ATF3 antibody (C-Term) (ABIN6256914)

Target: ATF3 (Activating Transcription Factor 3 (ATF3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-ATF3 Antibody

Binding Specificity: C-Term

Specificity: ATF3 Antibody detects endogenous levels of total ATF3.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ATF3, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ATF3

Target: ATF3 (Activating Transcription Factor 3 (ATF3))

Alternative Name: ATF3

Background:

Description: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.

Gene: ATF3

Molecular Weight: 21 kDa

Gene ID: 467

UniProt: P18847

Pathways: Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response