MOB1A antibody (N-Term)

Catalog No. ABIN6257009

This Rabbit Polyclonal antibody specifically detects MOB1A in WB, IF and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for MOB1A antibody (N-Term) (ABIN6257009)

Target: MOB1A (MOBKL1B) (MOB1, Mps One Binder Kinase Activator-Like 1B (MOBKL1B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MOB1A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MOB1A Antibody

Binding Specificity: N-Term

Purpose: MOB1A Antibody

Specificity: MOB1A Antibody detects endogenous levels of total MOB1A.

Predicted Reactivity: Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MOB1A, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MOB1A

Target: MOB1A (MOBKL1B) (MOB1, Mps One Binder Kinase Activator-Like 1B (MOBKL1B))

Alternative Name: MOB1A

Background:

Description: Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L.

Gene: MOB1B

Molecular Weight: 25 kDa

Gene ID: 92597

UniProt: Q7L9L4