HUCE1 (Internal Region) antibody
Quick Overview for HUCE1 (Internal Region) antibody (ABIN6257014)
Target
Reactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- Internal Region
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Purpose
- HUCE1 Antibody
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Specificity
- HUCE1 Antibody detects endogenous levels of total HUCE1.
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Predicted Reactivity
- Pig,Bovine,Horse,Sheep,Rabbit,Dog
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Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
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Immunogen
- A synthesized peptide derived from human HUCE1, corresponding to a region within the internal amino acids.
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Isotype
- IgG
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anti-HUCE1 (AA 271-320) antibody
Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated
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Application Notes
- WB 1:500-1:1000, IF/ICC 1:100-1:500
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
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Expiry Date
- 12 months
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- HUCE1
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Background
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Description: Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.
Gene: RRP8
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Molecular Weight
- 51 kDa
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Gene ID
- 23378
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UniProt
- O43159
Target
-
