PFKL antibody (C-Term)

Catalog No. ABIN6257021

This Rabbit Polyclonal antibody specifically detects PFKL in WB, ELISA, IHC, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PFKL antibody (C-Term) (ABIN6257021)

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PFKL Antibody

Binding Specificity: C-Term

Specificity: K6PL Antibody detects endogenous levels of total K6PL.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human K6PL, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for PFKL

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Alternative Name: PFKL

Background:

Description: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (PubMed:22923583). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (By similarity).

Gene: PFKL

Molecular Weight: 85 kDa

Gene ID: 5211

UniProt: P17858

Pathways: Negative Regulation of Hormone Secretion, Warburg Effect