ARHGAP17 antibody (Internal Region)

Catalog No. ABIN6257043

The Rabbit Polyclonal anti-ARHGAP17 antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect ARHGAP17 in samples from Human, Rat and Mouse.

Quick Overview for ARHGAP17 antibody (Internal Region) (ABIN6257043)

Target: ARHGAP17 (rho GTPase Activating Protein 17 (ARHGAP17))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGAP17 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ARHGAP17 Antibody

Binding Specificity: Internal Region

Specificity: RHG17 Antibody detects endogenous levels of total RHG17.

Predicted Reactivity: Bovine,Horse,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RHG17, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ARHGAP17

Target: ARHGAP17 (rho GTPase Activating Protein 17 (ARHGAP17))

Alternative Name: ARHGAP17

Background:

Description: Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. Participates in the Ca2+-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activator in vitro for RAC1.

Gene: ARHGAP17

Molecular Weight: 100 kDa

Gene ID: 55114

UniProt: Q68EM7