CDK5RAP3 antibody (Internal Region)

Catalog No. ABIN6257054

This Rabbit Polyclonal antibody specifically detects CDK5RAP3 in WB, IF and ICC. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for CDK5RAP3 antibody (Internal Region) (ABIN6257054)

Target: CDK5RAP3 (CDK5 Regulatory Subunit Associated Protein 3 (CDK5RAP3))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK5RAP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CDK5RAP3 Antibody

Binding Specificity: Internal Region

Purpose: CDK5RAP3 Antibody

Specificity: CDK5RAP3 Antibody detects endogenous levels of total CDK5RAP3

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CDK5RAP3, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CDK5RAP3

Target: CDK5RAP3 (CDK5 Regulatory Subunit Associated Protein 3 (CDK5RAP3))

Alternative Name: CDK5RAP3

Background:

Description: Probable tumor suppressor initially identified as a CDK5R1 interactor controlling cell proliferation (PubMed:12054757, PubMed:12737517). Negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation (PubMed:17785205, PubMed:20228063). Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint (PubMed:15790566, PubMed:19223857). Through its interaction with CDKN2A/ARF and MDM2 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization and activation in the nucleus, thereby promoting G1 cell cycle arrest and inhibition of cell proliferation (PubMed:16173922). May play a role in the unfolded protein response, mediating the ufmylation of multiple proteins in response to endoplasmic reticulum stress (PubMed:23152784). May also play a role in the rupture of the nuclear envelope during apoptosis (PubMed:23478299). May regulate MAPK14 activity by regulating its dephosphorylation by PPM1D/WIP1 (PubMed:21283629).

Gene: CDK5RAP3

Molecular Weight: 60 kDa

Gene ID: 80279

UniProt: Q96JB5