ATP5C1 antibody (Internal Region)

Catalog No. ABIN6257083

The Rabbit Polyclonal anti-ATP5C1 antibody (ABIN6257083) specifically detects ATP5C1 in WB, ELISA, IF and ICC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for ATP5C1 antibody (Internal Region) (ABIN6257083)

Target: ATP5C1 (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, gamma Polypeptide 1 (ATP5C1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5C1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ATP5C1 Antibody

Binding Specificity: Internal Region

Specificity: ATPG Antibody detects endogenous levels of total ATPG.

Predicted Reactivity: Bovine,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ATPG, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ATP5C1

Target: ATP5C1 (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, gamma Polypeptide 1 (ATP5C1))

Alternative Name: ATP5C1

Background:

Description: Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F1 domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha3beta3. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Gene: ATP5C1

Molecular Weight: 33 kDa

Gene ID: 509

UniProt: P36542

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process