DBF4 antibody (Internal Region)

Catalog No. ABIN6257125

The Rabbit Polyclonal anti-DBF4 antibody (ABIN6257125) specifically detects DBF4 in WB, ELISA, IF and ICC. The antibody is reactive with Human and Mouse samples.

Quick Overview for DBF4 antibody (Internal Region) (ABIN6257125)

Target: DBF4 (DBF4 Homolog (DBF4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DBF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-DBF4 Antibody

Binding Specificity: Internal Region

Specificity: DBF4 Antibody detects endogenous levels of total DBF4.

Predicted Reactivity: Pig,Horse,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human DBF4, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for DBF4

Target: DBF4 (DBF4 Homolog (DBF4))

Alternative Name: DBF4

Background:

Description: Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.

Gene: DBF4

Molecular Weight: 77 kDa

Gene ID: 10926

UniProt: Q9UBU7

Pathways: Mitotic G1-G1/S Phases, DNA Replication