DBF4 antibody (AA 10-90)

Catalog No. ABIN7214522

This Rabbit Polyclonal antibody specifically detects DBF4 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Monkey.

Quick Overview for DBF4 antibody (AA 10-90) (ABIN7214522)

Target: DBF4 (DBF4 Homolog (DBF4))

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DBF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-DBF4 Antibody

Binding Specificity: AA 10-90

Purpose: Rabbit Anti-Dbf4 Polyclonal Antibody

Specificity: Dbf4 Polyclonal Antibody detects endogenous levels of Dbf4 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Dbf4 at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DBF4

Target: DBF4 (DBF4 Homolog (DBF4))

Alternative Name: Dbf4

Background: DBF4, ASK, DBF4A, ZDBF1, Protein DBF4 homolog A, Activator of S phase kinase, Chiffon homolog A, DBF4-type zinc finger-containing protein 1The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase α-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosomes separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.

Molecular Weight: 77kD

Gene ID: 10926

UniProt: Q9UBU7

Pathways: Mitotic G1-G1/S Phases, DNA Replication