MDFI antibody (Internal Region)

Catalog No. ABIN6257357

This Rabbit Polyclonal antibody specifically detects MDFI in WB, ELISA, IHC, IF and ICC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for MDFI antibody (Internal Region) (ABIN6257357)

Target: MDFI (MyoD Family Inhibitor (MDFI))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDFI antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MDFI Antibody

Binding Specificity: Internal Region

Specificity: MDFI Antibody detects endogenous levels of total MDFI.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MDFI, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MDFI

Target: MDFI (MyoD Family Inhibitor (MDFI))

Alternative Name: MDFI

Background:

Description: Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways (By similarity).

Gene: MDFI

Molecular Weight: 25 kDa

Gene ID: 4188

UniProt: Q99750

Pathways: Maintenance of Protein Location