DOCK1 antibody (C-Term)

Catalog No. ABIN6257863

This anti-DOCK1 antibody is a Rabbit Polyclonal antibody detecting DOCK1 in ELISA, WB, IHC, IF and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for DOCK1 antibody (C-Term) (ABIN6257863)

Target: DOCK1 (Dedicator of Cytokinesis 1 (DOCK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOCK1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-DOCK1 Antibody

Binding Specificity: C-Term

Specificity: DOCK1 Antibody detects endogenous levels of total DOCK1.

Predicted Reactivity: Zebrafish,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human DOCK1, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for DOCK1

Target: DOCK1 (Dedicator of Cytokinesis 1 (DOCK1))

Alternative Name: DOCK1

Background:

Description: Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen (PubMed:19004829). Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1 (PubMed:8657152).

Gene: DOCK1

Molecular Weight: 215 kDa

Gene ID: 1793

UniProt: Q14185

Pathways: Signaling of Hepatocyte Growth Factor Receptor