RNF144B antibody (Internal Region)

Catalog No. ABIN6258475

Product Details anti-RNF144B Antibody

Target: RNF144B (Ring Finger Protein 144B (RNF144B))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF144B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: RNF144B Antibody detects endogenous levels of total RNF144B.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RNF144B, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for RNF144B

Target: RNF144B (Ring Finger Protein 144B (RNF144B))

Alternative Name: RNF144B (RNF144B Products)

Synonyms: IBRDC2 antibody, PIR2 antibody, bA528A10.3 antibody, p53RFP antibody, BC025007 antibody, E130105P19Rik antibody, Ibrdc2 antibody, Pir2 antibody, ring finger protein 144B antibody, RNF144B antibody, Rnf144b antibody

Background:

Description: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death, But, it is not an anti-apoptotic protein per se.

Gene: RNF144B

Molecular Weight: 33 kDa

Gene ID: 255488

UniProt: Q7Z419