CLIC4 antibody (N-Term)

Catalog No. ABIN625856

The Rabbit Polyclonal anti-CLIC4 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect CLIC4 in samples from Human, Mouse, Rat, Guinea Pig and Monkey.

Quick Overview for CLIC4 antibody (N-Term) (ABIN625856)

Target: CLIC4 (Chloride Intracellular Channel 4 (CLIC4))

Reactivity: Human, Mouse, Rat, Guinea Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLIC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CLIC4 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Human CLIC4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Dog, Bovine (92%).

Purification: Protein A purified

Immunogen: Synthetic peptide from N-Terminus of human CLIC4 (NP_039234). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Guinea pig (100%), Panda, Dog, Bovine (92%), Zebra finch (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (20 μg/mL), WB (2.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CLIC4

Target: CLIC4 (Chloride Intracellular Channel 4 (CLIC4))

Alternative Name: CLIC4

Background: Name/Gene ID: CLIC4
Subfamily: Chloride channel - intracellular
Family: Ion Channel

Synonyms: CLIC4, CLIC4L, DKFZP566G223, Mc3s5, HuH1, MTCLIC, p64H1

Gene ID: 25932

NCBI Accession: NP_039234

UniProt: Q9Y696