CD177 antibody (Internal Region)

Catalog No. ABIN6258621

The Rabbit Polyclonal anti-CD177 antibody is suitable to detect CD177 in samples from Human and Rat. It has been validated for WB, IHC and ELISA.

Quick Overview for CD177 antibody (Internal Region) (ABIN6258621)

Target: CD177 (CD177 Molecule (CD177))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD177 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-CD177 Antibody

Binding Specificity: Internal Region

Specificity: CD177 Antibody detects endogenous levels of total CD177.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CD177, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Expiry Date: 12 months

Target Details for CD177

Target: CD177 (CD177 Molecule (CD177))

Alternative Name: CD177

Background:

Description: In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (PubMed:21193407). In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (PubMed:28807980). Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (PubMed:17580308). However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed:23461681). Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (PubMed:17244676, PubMed:18462208). By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (PubMed:23202369).

Gene: CD177

Molecular Weight: ?46 kDa

Gene ID: 57126

UniProt: Q8N6Q3