TSEN54 antibody (Internal Region)

Catalog No. ABIN6258653

This Rabbit Polyclonal antibody specifically detects TSEN54 in WB, IHC, ELISA, ICC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for TSEN54 antibody (Internal Region) (ABIN6258653)

Target: TSEN54 (tRNA Splicing Endonuclease 54 (TSEN54))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TSEN54 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-TSEN54 Antibody

Binding Specificity: Internal Region

Specificity: TSEN54 Antibody detects endogenous levels of total TSEN54.

Predicted Reactivity: Bovine

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TSEN54, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for TSEN54

Target: TSEN54 (tRNA Splicing Endonuclease 54 (TSEN54))

Alternative Name: TSEN54

Background:

Description: Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events.

Gene: TSEN54

Molecular Weight: 59 kDa

Gene ID: 283989

UniProt: Q7Z6J9

Pathways: Synaptic Membrane