NK2 Homeobox 5 antibody (Internal Region)

Catalog No. ABIN6259127

This Rabbit Polyclonal antibody specifically detects NK2 Homeobox 5 in WB, ICC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NK2 Homeobox 5 antibody (Internal Region) (ABIN6259127)

Target: NK2 Homeobox 5 (NKX2-5)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NK2 Homeobox 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-NK2 Homeobox 5 Antibody

Binding Specificity: Internal Region

Purpose: Nkx2.5 Antibody

Specificity: Nkx2.5 Antibody detects endogenous levels of total Nkx2.5.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Nkx2.5, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for NK2 Homeobox 5

Target: NK2 Homeobox 5 (NKX2-5)

Alternative Name: Nkx2.5

Background:

Description: Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). Binds to the core DNA motif of NPPA promoter (PubMed:22849347, PubMed:26926761). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development.

Gene: NKX2-5

Molecular Weight: 50 kDa

Gene ID: 1482

UniProt: P52952

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development