NFE2L1 antibody (N-Term)

Catalog No. ABIN6259245

The Rabbit Polyclonal anti-NFE2L1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect NFE2L1 in samples from Human and Mouse.

Quick Overview for NFE2L1 antibody (N-Term) (ABIN6259245)

Target: NFE2L1 (Nuclear Factor (erythroid-Derived 2)-Like 1 (NFE2L1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFE2L1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-NFE2L1 Antibody

Binding Specificity: N-Term

Specificity: NFE2L1 Antibody detects endogenous levels of total NFE2L1.

Predicted Reactivity: Pig,Bovine,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human NFE2L1, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Expiry Date: 12 months

Target Details for NFE2L1

Target: NFE2L1 (Nuclear Factor (erythroid-Derived 2)-Like 1 (NFE2L1))

Alternative Name: NFE2L1

Background:

Description: Endoplasmic reticulum membrane sensor NFE2L1: Endoplasmic reticulum membrane sensor that translocates into the nucleus in response to various stresses to act as a transcription factor (PubMed:20932482, PubMed:24448410). Constitutes a precursor of the transcription factor NRF1 (By similarity). Able to detect various cellular stresses, such as cholesterol excess, oxidative stress or proteasome inhibition (PubMed:20932482). In response to stress, it is released from the endoplasmic reticulum membrane following cleavage by the protease DDI2 and translocates into the nucleus to form the transcription factor NRF1 (By similarity). Acts as a key sensor of cholesterol excess: in excess cholesterol conditions, the endoplasmic reticulum membrane form of the protein directly binds cholesterol via its CRAC motif, preventing cleavage and release of the transcription factor NRF1, thereby allowing expression of genes promoting cholesterol removal, such as CD36 (By similarity). Involved in proteasome homeostasis: in response to proteasome inhibition, it is released from the endoplasmic reticulum membrane, translocates to the nucleus and activates expression of genes encoding proteasome subunits (PubMed:20932482).

Gene: NFE2L1

Molecular Weight: 82 kDa

Gene ID: 4779

UniProt: Q14494