RRAGA/B antibody

Catalog No. ABIN6259340

Product Details anti-RRAGA/B Antibody

Target: RRAGA/B (Ras-Related GTP Binding A/B (RRAGA/B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RRAGA/B antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: RRAGA/B Antibody detects endogenous levels of total RRAGA/B

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide

Isotype: IgG

Application Details

Application Notes: WB 1:500~1:1000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for RRAGA/B

Target: RRAGA/B (Ras-Related GTP Binding A/B (RRAGA/B))

Alternative Name: RRAGA/B (RRAGA/B Products)

Background:

Description: Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGC or RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death.

Gene: RRAGA

Molecular Weight: 34 kDa

Gene ID: 10670

UniProt: Q7L523, Q5VZM2