RFTN1 antibody (Internal Region)

Catalog No. ABIN6259466

This Rabbit Polyclonal antibody specifically detects RFTN1 in WB, ICC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RFTN1 antibody (Internal Region) (ABIN6259466)

Target: RFTN1 (Raftlin, Lipid Raft Linker 1 (RFTN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFTN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-RFTN1 Antibody

Binding Specificity: Internal Region

Purpose: RFTN1 Antibody

Specificity: RFTN1 Antibody detects endogenous levels of total RFTN1.

Predicted Reactivity: Horse,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RFTN1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for RFTN1

Target: RFTN1 (Raftlin, Lipid Raft Linker 1 (RFTN1))

Alternative Name: RFTN1

Background:

Description: Involved in protein trafficking via association with clathrin and AP2 complex (PubMed:27022195, PubMed:21266579). Upon bacterial lipopolysaccharide stimulation, mediates internalization of TLR4 to endosomes in dendritic cells and macrophages, and internalization of poly(I:C) to TLR3-positive endosomes in myeloid dendritic cells and epithelial cells, resulting in activation of TICAM1-mediated signaling and subsequent IFNB1 production (PubMed:27022195, PubMed:21266579). Involved in T-cell antigen receptor-mediated signaling by regulating tyrosine kinase LCK localization, T-cell dependent antibody production and cytokine secretion (By similarity). May regulate B-cell antigen receptor-mediated signaling (PubMed:12805216). May play a pivotal role in the formation and/or maintenance of lipid rafts (PubMed:12805216).

Gene: RFTN1

Molecular Weight: 63 kDa

Gene ID: 23180

UniProt: Q14699