WEE2 antibody (Internal Region)

Catalog No. ABIN6259536

The Rabbit Polyclonal anti-WEE2 antibody is suitable to detect WEE2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ICC and IF.

Quick Overview for WEE2 antibody (Internal Region) (ABIN6259536)

Target: WEE2 (WEE1 Homolog 2 (S. Pombe) (WEE2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WEE2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-WEE2 Antibody

Binding Specificity: Internal Region

Purpose: WEE2 Antibody

Specificity: WEE2 Antibody detects endogenous levels of total WEE2

Predicted Reactivity: Pig,Horse,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human WEE2, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for WEE2

Target: WEE2 (WEE1 Homolog 2 (S. Pombe) (WEE2))

Alternative Name: WEE2

Background:

Description: Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation (By similarity).

Gene: WEE2

Molecular Weight: 60 kDa

Gene ID: 494551

UniProt: P0C1S8

Pathways: M Phase