WEE2 antibody

Catalog No. ABIN6259536

Product Details anti-WEE2 Antibody

Target: WEE2 (WEE1 Homolog 2 (S. Pombe) (WEE2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WEE2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: WEE2 Antibody detects endogenous levels of total WEE2

Cross-Reactivity: Human, Mouse (Murine)

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide

Isotype: IgG

Application Details

Application Notes: WB 1:500~1:1000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for WEE2

Target: WEE2 (WEE1 Homolog 2 (S. Pombe) (WEE2))

Alternative Name: WEE2 (WEE2 Products)

Synonyms: Xe-wee1 antibody, wee1 antibody, wee1-b antibody, wee1a antibody, wee1b antibody, wee2-b antibody, xwee1 antibody, xwee2 antibody, WEE1B antibody, Gm1065 antibody, Wee1B antibody, WEE2 antibody, im:5913035 antibody, si:ch211-238n5.5 antibody, wu:fi13b10 antibody, zgc:123074 antibody, WEE1 homolog 2 antibody, WEE1 homolog 2 (S. pombe) antibody, wee2 antibody, WEE2 antibody, Wee2 antibody

Background:

Description: Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation (By similarity).

Gene: WEE2

Molecular Weight: 60 kDa

Gene ID: 494551

UniProt: P0C1S8

Pathways: M Phase