NEMF antibody (C-Term)

Catalog No. ABIN6259616

This Rabbit Polyclonal antibody specifically detects NEMF in WB, ELISA, ICC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NEMF antibody (C-Term) (ABIN6259616)

Target: NEMF (SDCCAG1) (serologically Defined Colon Cancer Antigen 1 (SDCCAG1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEMF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-NEMF Antibody

Binding Specificity: C-Term

Specificity: SDCG1 Antibody detects endogenous levels of total SDCG1.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human SDCG1, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for NEMF

Target: NEMF (SDCCAG1) (serologically Defined Colon Cancer Antigen 1 (SDCCAG1))

Alternative Name: NEMF

Background:

Description: Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. NEMF is responsible for selective recognition of stalled 60S subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety. NEMF is important for the stable association of LTN1 to the complex (PubMed:25578875). May indirectly play a role in nuclear export (PubMed:16103875).

Gene: NEMF

Molecular Weight: 123 kDa

Gene ID: 9147

UniProt: O60524