NEMF antibody (AA 850-930)

Catalog No. ABIN7216148

The Rabbit Polyclonal anti-NEMF antibody is suitable to detect NEMF in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for NEMF antibody (AA 850-930) (ABIN7216148)

Target: NEMF (SDCCAG1) (serologically Defined Colon Cancer Antigen 1 (SDCCAG1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEMF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-NEMF Antibody

Binding Specificity: AA 850-930

Purpose: Rabbit Anti-NY-CO-1 Polyclonal Antibody

Specificity: NY-CO-1 Polyclonal Antibody detects endogenous levels of NY-CO-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human NY-CO-1 at AA range: 850-930

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NEMF

Target: NEMF (SDCCAG1) (serologically Defined Colon Cancer Antigen 1 (SDCCAG1))

Alternative Name: NY-CO-1

Background: NEMF, SDCCAG1, Nuclear export mediator factor NEMF, Antigen NY-CO-1, Serologically defined colon cancer antigen 1NEMF encodes a component of the ribosome quality control complex. Nuclear export mediator factor facilitates the recognition and ubiquitination of stalled 60S subunits by the ubiquitin ligase listerin. A similar protein in fly functions as a tumor suppressor.

Molecular Weight: 123kD

Gene ID: 9147

UniProt: O60524