AFG3L2 antibody (C-Term)

Catalog No. ABIN6259816

This anti-AFG3L2 antibody is a Rabbit Polyclonal antibody detecting AFG3L2 in WB and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for AFG3L2 antibody (C-Term) (ABIN6259816)

Target: AFG3L2 (AFG3-Like Protein 2 (AFG3L2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFG3L2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-AFG3L2 Antibody

Binding Specificity: C-Term

Specificity: AFG3L2 Antibody detects endogenous levels of total AFG3L2.

Predicted Reactivity: Pig,Bovine,Sheep,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human AFG3L2, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for AFG3L2

Target: AFG3L2 (AFG3-Like Protein 2 (AFG3L2))

Alternative Name: AFG3L2

Background:

Description: ATP-dependent protease which is essential for axonal and neuron development. In neurons, mediates degradation of SMDT1/EMRE before its assembly with the uniporter complex, limiting the availability of SMDT1/EMRE for MCU assembly and promoting efficient assembly of gatekeeper subunits with MCU (PubMed:27642048). Required for the maturation of paraplegin (SPG7) after its cleavage by mitochondrial-processing peptidase (MPP), converting it into a proteolytically active mature form (By similarity).

Gene: AFG3L2

Molecular Weight: 80-90 kDa

Gene ID: 10939

UniProt: Q9Y4W6

Pathways: Skeletal Muscle Fiber Development