AKAP9 antibody (C-Term)

Catalog No. ABIN6259858

This anti-AKAP9 antibody is a Rabbit Polyclonal antibody detecting AKAP9 in ELISA, WB, IF and ICC. Suitable for Human.

Quick Overview for AKAP9 antibody (C-Term) (ABIN6259858)

Target: AKAP9 (A Kinase (PRKA) Anchor Protein (Yotiao) 9 (AKAP9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKAP9 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-AKAP9 Antibody

Binding Specificity: C-Term

Specificity: AKAP9 Antibody detects endogenous levels of total AKAP9.

Predicted Reactivity: Pig,Bovine,Sheep,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human AKAP9, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for AKAP9

Target: AKAP9 (A Kinase (PRKA) Anchor Protein (Yotiao) 9 (AKAP9))

Alternative Name: AKAP9

Background:

Description: Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. Required to maintain the integrity of the Golgi apparatus (PubMed:10202149, PubMed:15047863). Required for microtubule nucleation at the cis-side of the Golgi apparatus (PubMed:15047863, PubMed:19242490). Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase (PubMed:25657325). In complex with PDE4DIP isoform 13/MMG8/SMYLE, recruits CAMSAP2 to the Golgi apparatus and tethers non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:27666745, PubMed:28814570). In complex with PDE4DIP isoform 13/MMG8/SMYLE, EB1/MAPRE1 and CDK5RAP2, contributes to microtubules nucleation and extension also from the centrosome to the cell periphery (PubMed:29162697).

Gene: AKAP9

Molecular Weight: 454kDa

Gene ID: 10142

UniProt: Q99996

Pathways: M Phase, SARS-CoV-2 Protein Interactome