Calpain 2 antibody (N-Term)

Catalog No. ABIN6260448

The Rabbit Polyclonal anti-Calpain 2 antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect Calpain 2 in samples from Human, Mouse and Rat.

Quick Overview for Calpain 2 antibody (N-Term) (ABIN6260448)

Target: Calpain 2 (CAPN2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calpain 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Calpain 2 Antibody

Binding Specificity: N-Term

Specificity: Calpain 2 Antibody detects endogenous levels of total Calpain 2.

Predicted Reactivity: Bovine,Sheep,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Calpain 2, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for Calpain 2

Target: Calpain 2 (CAPN2)

Alternative Name: CAPN2

Background:

Description: Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508). Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (By similarity).

Gene: CAPN2

Molecular Weight: 80kDa

Gene ID: 824

UniProt: P17655