CD34 antibody (Internal Region)

Catalog No. ABIN6260615

This Rabbit Polyclonal antibody specifically detects CD34 in WB, IF, ELISA and ICC. It exhibits reactivity toward Human, Rat and Mouse and has been mentioned in 2+ publications.

Quick Overview for CD34 antibody (Internal Region) (ABIN6260615)

Target: CD34

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

Product Details anti-CD34 Antibody

Binding Specificity: Internal Region

Specificity: CD34 Antibody detects endogenous levels of total CD34.

Predicted Reactivity: Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CD34, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:200, IF/ICC 1:0-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-CD34 antibody (ABIN6260615)

Ai, Zhang, Gao, Qin, Zhong, Gu, Li, Qiao, Tian, Cui, Yang, Bi, Xiao, Chen, Liu, Zhou, Sun, Yang: "Sesquiterpene binding Gly-Leu-Ser/Lys-"co-adaptation pocket" to inhibit lung cancer cell epithelial-mesenchymal transition." in: Oncotarget, Vol. 8, Issue 41, pp. 70192-70203, (2018) (PubMed).

Bai, Wang, Han, Xie, Ji, Yin, Chen, Wang, Jiang, Qi, Jiang: "BCL2L10 inhibits growth and metastasis of hepatocellular carcinoma both in vitro and in vivo." in: Molecular carcinogenesis, Vol. 56, Issue 3, pp. 1137-1149, (2017) (PubMed).

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background:

Description: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Gene: CD34

Molecular Weight: 120 kDa

Gene ID: 947

UniProt: P28906