CD57 antibody (N-Term)

Catalog No. ABIN6260635

This anti-CD57 antibody is a Rabbit Polyclonal antibody detecting CD57 in IHC, WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for CD57 antibody (N-Term) (ABIN6260635)

Target: CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD57 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Product Details anti-CD57 Antibody

Binding Specificity: N-Term

Specificity: CD57 Antibody detects endogenous levels of total CD57.

Predicted Reactivity: Horse,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CD57, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: IHC 1:50-1:200, WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CD57

Target: CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))

Alternative Name: B3GAT1

Background:

Description: Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.

Gene: B3GAT1

Molecular Weight: 38 kDa

Gene ID: 27087

UniProt: Q9P2W7

Pathways: Glycosaminoglycan Metabolic Process