CDK3 antibody (C-Term)

Catalog No. ABIN6260712

The Rabbit Polyclonal anti-CDK3 antibody has been validated for WB, ELISA, ICC and IF. It is suitable to detect CDK3 in samples from Human and Mouse. There is 1 publication available.

Quick Overview for CDK3 antibody (C-Term) (ABIN6260712)

Target: CDK3 (Cyclin-Dependent Kinase 3 (CDK3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-CDK3 Antibody

Binding Specificity: C-Term

Specificity: CDK3 Antibody detects endogenous levels of total CDK3.

Predicted Reactivity: Pig,Bovine,Sheep,Rabbit

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CDK3, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-CDK3 antibody (ABIN6260712)

Wang, Xi, Sun, Zhang, Jiang, He, Li: "CDK3 is a major target of miR-150 in cell proliferation and anti-cancer effect." in: Experimental and molecular pathology, Vol. 102, Issue 2, pp. 181-190, (2017) (PubMed).

Target Details for CDK3

Target: CDK3 (Cyclin-Dependent Kinase 3 (CDK3))

Alternative Name: CDK3

Background:

Description: Serine/threonine-protein kinase that plays a critical role in the control of the eukaryotic cell cycle, involved in G0-G1 and G1-S cell cycle transitions. Interacts with CCNC/cyclin-C during interphase. Phosphorylates histone H1, ATF1, RB1 and CABLES1. ATF1 phosphorylation triggers ATF1 transactivation and transcriptional activities, and promotes cell proliferation and transformation. CDK3/cyclin-C mediated RB1 phosphorylation is required for G0-G1 transition. Promotes G1-S transition probably by contributing to the activation of E2F1, E2F2 and E2F3 in a RB1-independent manner.

Gene: CDK3

Molecular Weight: 35 kDa

Gene ID: 1018

UniProt: Q00526

Pathways: Cell Division Cycle