CHD1 antibody (C-Term)

Catalog No. ABIN6260793

The Rabbit Polyclonal anti-CHD1 antibody has been validated for WB and ELISA. It is suitable to detect CHD1 in samples from Human and Mouse.

Quick Overview for CHD1 antibody (C-Term) (ABIN6260793)

Target: CHD1 (Chromodomain Helicase DNA Binding Protein 1 (CHD1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CHD1 Antibody

Binding Specificity: C-Term

Specificity: CHD1 Antibody detects endogenous levels of total CHD1.

Predicted Reactivity: Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CHD1, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CHD1

Target: CHD1 (Chromodomain Helicase DNA Binding Protein 1 (CHD1))

Alternative Name: CHD1

Background:

Description: ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity (By similarity). Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3 (PubMed:18042460, PubMed:28866611). Required for maintaining open chromatin and pluripotency in embryonic stem cells (By similarity).

Gene: CHD1

Molecular Weight: 197kDa

Gene ID: 1105

UniProt: O14646

Pathways: Chromatin Binding