DAPK2 antibody (C-Term)

Catalog No. ABIN6261222

The Rabbit Polyclonal anti-DAPK2 antibody is suitable to detect DAPK2 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC, IF and ICC.

Quick Overview for DAPK2 antibody (C-Term) (ABIN6261222)

Target: DAPK2 (Death-Associated Protein Kinase 2 (DAPK2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAPK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-DAPK2 Antibody

Binding Specificity: C-Term

Specificity: DAPK2 Antibody detects endogenous levels of total DAPK2.

Predicted Reactivity: Pig,Horse,Sheep,Rabbit

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human DAPK2, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:200-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for DAPK2

Target: DAPK2 (Death-Associated Protein Kinase 2 (DAPK2))

Alternative Name: DAPK2

Background:

Description: Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell death signals, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation (PubMed:17347302). Regulates granulocytic motility by controlling cell spreading and polarization (PubMed:24163421).

Gene: DAPK2

Molecular Weight: 42 kDa

Gene ID: 23604

UniProt: Q9UIK4