DNAJB14 antibody (C-Term)

Catalog No. ABIN6261329

Product Details anti-DNAJB14 Antibody

Target: DNAJB14 (DnaJ (Hsp40) Homolog, Subfamily B, Member 14 (DNAJB14))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJB14 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: DNAJB14 Antibody detects endogenous levels of total DNAJB14.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human DNAJB14, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for DNAJB14

Target: DNAJB14 (DnaJ (Hsp40) Homolog, Subfamily B, Member 14 (DNAJB14))

Alternative Name: DNAJB14 (DNAJB14 Products)

Synonyms: Dnajb14 antibody, MGC153638 antibody, DKFZp468H1319 antibody, EGNR9427 antibody, PRO34683 antibody, 5730496F10Rik antibody, flj14281 antibody, DnaJ heat shock protein family (Hsp40) member B14 antibody, DnaJ (Hsp40) homolog, subfamily B, member 14 antibody, DnaJ heat shock protein family (Hsp40) member B14 S homeolog antibody, DNAJB14 antibody, dnajb14 antibody, Dnajb14 antibody, dnajb14.S antibody

Background:

Description: Acts as a co-chaperone with HSPA8/Hsc70, required to promote protein folding and trafficking, prevent aggregation of client proteins, and promote unfolded proteins to endoplasmic reticulum-associated degradation (ERAD) pathway (PubMed:24732912). Acts by determining HSPA8/Hsc70's ATPase and polypeptide-binding activities (PubMed:24732912). Can also act independently of HSPA8/Hsc70: together with DNAJB12, acts as a chaperone that promotes maturation of potassium channels KCND2 and KCNH2 by stabilizing nascent channel subunits and assembling them into tetramers (PubMed:27916661). While stabilization of nascent channel proteins is dependent on HSPA8/Hsc70, the process of oligomerization of channel subunits is independent of HSPA8/Hsc70 (PubMed:27916661). When overexpressed, forms membranous structures together with DNAJB12 and HSPA8/Hsc70 within the nucleus, the role of these structures, named DJANGOs, is still unclear (PubMed:24732912).

Gene: DNAJB14

Molecular Weight: 43 kDa

Gene ID: 79982

UniProt: Q8TBM8