E2F6 antibody (Internal Region)

Catalog No. ABIN6261413

This Rabbit Polyclonal antibody specifically detects E2F6 in WB, IHC, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.It has been mentioned in 1 publication

Quick Overview for E2F6 antibody (Internal Region) (ABIN6261413)

Target: E2F6 (E2F Transcription Factor 6 (E2F6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-E2F6 Antibody

Binding Specificity: Internal Region

Purpose: E2F6 Antibody

Specificity: E2F6 Antibody detects endogenous levels of total E2F6.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human E2F6, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-E2F6 antibody (ABIN6261413)

Ai, Zhang, Gao, Qin, Zhong, Gu, Li, Qiao, Tian, Cui, Yang, Bi, Xiao, Chen, Liu, Zhou, Sun, Yang: "Sesquiterpene binding Gly-Leu-Ser/Lys-"co-adaptation pocket" to inhibit lung cancer cell epithelial-mesenchymal transition." in: Oncotarget, Vol. 8, Issue 41, pp. 70192-70203, (2018) (PubMed).

Target Details for E2F6

Target: E2F6 (E2F Transcription Factor 6 (E2F6))

Alternative Name: E2F6

Background:

Description: Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.

Gene: E2F6

Molecular Weight: 31kDa

Gene ID: 1876

UniProt: O75461