SR Proteins antibody

Catalog No. ABIN626151

The Mouse Monoclonal anti-SR Proteins antibody (ABIN626151) specifically detects SR Proteins in IF, ELISA, WB, IHC and IHC (p). The antibody is reactive with Human, Cow, Mouse, Rabbit and Rat samples.

Quick Overview for SR Proteins antibody (ABIN626151)

Target: SR Proteins

Reactivity: Human, Cow, Mouse, Rabbit, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Specificity: Detects a sub-set of the non-snRNP splicing factors termed SR proteins. The antibody detects SRp75, SRp55, SRp40 and SRp20 but not SRp30a or b (ASF/SF2, SC-35) proteins. Recognizes approximately 20 distinct nuclear proteins including the U1 70K component of the U1 snRNP and both subunits of U2AF1. Species cross-reactivity (Western Blot): chicken, human, mouse, rabbit, canine, rat, Xenopus. Reactivity has not been detected with yeasts.

Purification: Protein A purified

Immunogen: Purified, de-phosphorylated bovine Srp55 and Drosophila p55-GST.

Type of Immunogen: GST fusion protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (0.5 - 2 μg/mL), IHC, IHC-P (10 μg/mL), WB (3 - 5 μg/mL)

Usage: Suitable for use in Immunofluorescence, ELISA, Western Blot. Reactivity is confirmed by Western Blot. ELISA: 0.1-1 μg/mL. Immunofluorescence: 0.5-2 μg/mL, human. Western Blot: 3-5 μg/mL. Positive control: HeLa cell, nuclear extracts.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details

Target: SR Proteins