GJB2 antibody (Cytoplasmic Domain)

Catalog No. ABIN626152

This Mouse Monoclonal antibody specifically detects GJB2 in WB, ELISA, IHC, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GJB2 antibody (Cytoplasmic Domain) (ABIN626152)

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GJB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: CX-12H10

Product Details anti-GJB2 Antibody

Binding Specificity: Cytoplasmic Domain

Brand: IHC-plus™

Specificity: Reacts strongly with Connexin-26, which has a predicted molecular weight of 26.5kD. Although the polyclonal anti-Cx26 antibody (feline. No. 71-0500) raised against the same sequence as this monoclonal anti-Cx26 antibody cross-reacts with Cx30, this monoclonal anti-Cx26 appears to exhibit minimal cross-reaction with Cx30 by western blotting. The degree of cross-reaction with Cx30 by IHC is uncertain, but may be influenced by fixation conditions. Rat, mouse and human. Reactivity of this antibody on Western blots has been confirmed by using extracts from mouse liver, mouse brain and rat brain.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to a segment of the cytoplasmic loop of rat Connexin-26. (4,5).

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-Fr, IHC-P (10 μg/mL), WB (1 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1 μg/mL. Immunohistochemistry (Frozen): 1-2 μg/mL. Western Blot: 1 μg/mL. Reactivity confirmed using extracts from mouse liver, mouse brain and rat brain.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for GJB2

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Alternative Name: GJB2 / CX26 / Connexin 26

Background: Name/Gene ID: GJB2
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJB2, Connexin 26, Connexin-26, DFNB1A, DFNA3, DFNB1, Gap junction protein beta 2, KID, HID, PPK, NSRD1, CX26, DFNA3A

Gene ID: 2706

UniProt: P29033

Pathways: Sensory Perception of Sound, Cell-Cell Junction Organization