Ephrin B3 antibody (Internal Region)

Catalog No. ABIN6261572

This Rabbit Polyclonal antibody specifically detects Ephrin B3 in WB, ELISA, IHC, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for Ephrin B3 antibody (Internal Region) (ABIN6261572)

Target: Ephrin B3 (EFNB3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ephrin B3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Ephrin B3 Antibody

Binding Specificity: Internal Region

Specificity: Ephrin-B3 Antibody detects endogenous levels of total Ephrin-B3.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Ephrin-B3, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for Ephrin B3

Target: Ephrin B3 (EFNB3)

Alternative Name: EFNB3

Background:

Description: Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).

Gene: EFNB3

Molecular Weight: 36kDa

Gene ID: 1949

UniProt: Q15768

Pathways: RTK Signaling