ERAP1 antibody (Internal Region)

Catalog No. ABIN6261584

This Rabbit Polyclonal antibody specifically detects ERAP1 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for ERAP1 antibody (Internal Region) (ABIN6261584)

Target: ERAP1 (Endoplasmic Reticulum Aminopeptidase 1 (ERAP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ERAP1 Antibody

Binding Specificity: Internal Region

Specificity: ERAP1 Antibody detects endogenous levels of total ERAP1.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ERAP1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ERAP1

Target: ERAP1 (Endoplasmic Reticulum Aminopeptidase 1 (ERAP1))

Alternative Name: ERAP1

Background:

Description: Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.

Gene: ERAP1

Molecular Weight: 107 kDa

Gene ID: 51752

UniProt: Q9NZ08