FOXN1 antibody (N-Term)

Catalog No. ABIN6261798

The Rabbit Polyclonal anti-FOXN1 antibody has been validated for WB, ELISA, ICC and IF. It is suitable to detect FOXN1 in samples from Human and Rat.

Quick Overview for FOXN1 antibody (N-Term) (ABIN6261798)

Target: FOXN1 (Forkhead Box N1 (FOXN1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-FOXN1 Antibody

Binding Specificity: N-Term

Specificity: FOXN1 Antibody detects endogenous levels of total FOXN1.

Predicted Reactivity: Pig,Bovine,Horse,Rabbit

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human FOXN1, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for FOXN1

Target: FOXN1 (Forkhead Box N1 (FOXN1))

Alternative Name: FOXN1

Background:

Description: Transcriptional regulator which regulates the development, differentiation, and function of thymic epithelial cells (TECs) both in the prenatal and postnatal thymus. Acts as a master regulator of the TECs lineage development and is required from the onset of differentiation in progenitor TECs in the developing fetus to the final differentiation steps through which TECs mature to acquire their full functionality. Regulates, either directly or indirectly the expression of a variety of genes that mediate diverse aspects of thymus development and function, including MHC Class II, DLL4, CCL25, CTSL, CD40 and PAX1. Regulates the differentiation of the immature TECs into functional cortical TECs (cTECs) and medullary TECs (mTECs). Essential for maintenance of mTECs population in the postnatal thymus. Involved in the morphogenesis and maintenance of the three-dimensional thymic microstructure which is necessary for a fully functional thymus. Plays an important role in the maintenance of hematopoiesis and particularly T lineage progenitors within the bone marrow niche with age. Essential for the vascularization of the thymus anlage. Promotes the terminal differentiation of epithelial cells in the epidermis and hair follicles, partly by negatively regulating the activity of protein kinase C (By similarity). Plays a crucial role in the early prenatal stages of T-cell ontogeny (PubMed:21507891).

Gene: FOXN1

Molecular Weight: 69kDa

Gene ID: 8456

UniProt: O15353