GLI2 antibody (N-Term)

Catalog No. ABIN6261972

The Rabbit Polyclonal anti-GLI2 antibody is suitable to detect GLI2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, IF and ICC.

Quick Overview for GLI2 antibody (N-Term) (ABIN6261972)

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-GLI2 Antibody

Binding Specificity: N-Term

Specificity: Gli2 Antibody detects endogenous levels of total Gli2.

Predicted Reactivity: Pig,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Gli2, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for GLI2

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Alternative Name: GLI2

Background:

Description: Functions as transcription regulator in the hedgehog (Hh) pathway (PubMed:18455992, PubMed:26565916). Functions as transcriptional activator (PubMed:9557682, PubMed:19878745, PubMed:24311597). May also function as transcriptional repressor (By similarity). Requires STK36 for full transcriptional activator activity. Required for normal embryonic development (PubMed:15994174, PubMed:20685856).

Gene: GLI2

Molecular Weight: 133 kDa

Gene ID: 2736

UniProt: P10070

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis