Glypican 3 antibody (N-Term)

Catalog No. ABIN6262041

The Rabbit Polyclonal anti-Glypican 3 antibody has been validated for IF, WB, IHC, ELISA and ICC. It is suitable to detect Glypican 3 in samples from Human, Rat and Mouse.

Quick Overview for Glypican 3 antibody (N-Term) (ABIN6262041)

Target: Glypican 3 (GPC3)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glypican 3 antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Product Details anti-Glypican 3 Antibody

Binding Specificity: N-Term

Specificity: GPC3 Antibody detects endogenous levels of total GPC3.

Predicted Reactivity: Zebrafish,Bovine,Horse,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human GPC3, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for Glypican 3

Target: Glypican 3 (GPC3)

Alternative Name: GPC3

Background:

Description: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.

Gene: GPC3

Molecular Weight: 66kDa

Gene ID: 2719

UniProt: P51654

Pathways: Glycosaminoglycan Metabolic Process