HECTD1 antibody

Catalog No. ABIN6262200

The Rabbit Polyclonal anti-HECTD1 antibody has been validated for WB and ELISA. It is suitable to detect HECTD1 in samples from Human, Mouse and Rat.

Quick Overview for HECTD1 antibody (ABIN6262200)

Target: HECTD1 (HECT Domain Containing 1 (HECTD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HECTD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HECTD1 Antibody

Specificity: HECTD1 Antibody detects endogenous levels of total HECTD1

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human HECTD1

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for HECTD1

Target: HECTD1 (HECT Domain Containing 1 (HECTD1))

Alternative Name: HECTD1

Background:

Description: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates 'Lys-63'-linked polyubiquitination of HSP90AA1 which leads to its intracellular localization and reduced secretion. Negatively regulating HSP90AA1 secretion in cranial mesenchyme cells may impair their emigration and may be essential for the correct development of the cranial neural folds and neural tube closure.

Gene: HECTD1

Molecular Weight: 289kDa

Gene ID: 25831

UniProt: Q9ULT8

Pathways: Tube Formation, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection