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HSP27 antibody (Internal Region)

Name: HSP27 antibody (Internal Region)
SKU: ABIN6262392
Price: 494.77 USD
Availability: InStock

HSPB1 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

Western blot analysis of HSP27 expression in Ca2+ treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (HSP27 antibody (Internal Region))

ABIN6269087 at 1/100 staining Mouse pancreas tissue by IHC-P. (HSP27 antibody (Internal Region))

IHC

IF/ICC

3 images

(1 reference)

Catalog No. ABIN6262392

Datasheet as PDF

Target See all HSP27 (HSPB1) Antibodies

HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
Binding Specificity
40

32

31

16

16

8

8

6

6

6

5

4

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Internal Region
Reactivity
293

99

86

19

13

13

13

10

7

5

4

3

3

2

1

1
Human, Mouse, Rat
Host
232

66
Rabbit
Compatible Secondaries
Clonality
206

91
Polyclonal
Conjugate
131

20

15

11

10

7

5

5

5

5

5

5

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

2

1

1

1

1

1

1

1

1
This HSP27 antibody is un-conjugated
Application
243

103

100

87

64

48

46

42

41

40

15

12

10

7

7

3

2

2

2

2

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity

HSP27 Antibody detects endogenous levels of total HSP27.

Predicted Reactivity

Pig,Zebrafish,Bovine,Horse,Rabbit,Dog,Xenopus

Purification

The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen

A synthesized peptide derived from human HSP27, corresponding to a region within the internal amino acids.

Isotype

IgG

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HSPB1 Reactivity: Human WB, IHC, ELISA, FACS, ICC Host: Mouse Monoclonal 5D7 unconjugated

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HSPB1 Reactivity: Human WB, IHC, ELISA, IF, FACS, IP Host: Rabbit Monoclonal 10C11 unconjugated Recombinant Antibody

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HSPB1 Reactivity: Human WB, IHC, IF, FACS, IP, ICC Host: Mouse Monoclonal 8A7 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 Reactivity: Human, Mouse, Rat, Sheep, Chimpanzee, Chicken, Monkey WB, IHC, IF, FACS, ISt, StM Host: Mouse Monoclonal G3-1 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibody
HSPB1 Reactivity: Human, Mouse, Rat WB, IF, FACS, IHC (p), IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 96-205) antibody
HSPB1 Reactivity: Human WB, ELISA, IF, IP, PLA Host: Mouse Monoclonal 3G3 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 Reactivity: Human WB, IHC, IF, FACS, ICC Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 Reactivity: Human WB, IHC, ELISA, IF, IP, ICC Host: Mouse Monoclonal 5D12-A12 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (HRP)
HSPB1 Reactivity: Human WB, IHC, IF, FACS, IP, ICC Host: Mouse Monoclonal 8A7 HRP

Application Notes

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date

12 months
Xia, Yao, Tang, Xiao, Yang, Zhou: "Isobaric Tags for Relative and Absolute Quantitation (iTRAQ)-Based Proteomic Analysis of Hugan Qingzhi and Its Protective Properties against Free Fatty Acid-Induced L02 Hepatocyte Injury." in: Frontiers in pharmacology, Vol. 8, pp. 99, (2017) (PubMed).
Target

HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name

HSPB1 (HSPB1 Products)

Background

Description: Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).
Gene: HSPB1

Molecular Weight

27kDa

Gene ID

3315

UniProt

P04792

Pathways

MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling