HUS1 antibody (Internal Region)

Catalog No. ABIN6262423

Product Details anti-HUS1 Antibody

Target: HUS1 (HUS1 Checkpoint Homolog (S. Pombe) (HUS1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HUS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: HUS1 Antibody detects endogenous levels of total HUS1.

Predicted Reactivity: Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human HUS1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for HUS1

Target: HUS1 (HUS1 Checkpoint Homolog (S. Pombe) (HUS1))

Alternative Name: HUS1 (HUS1 Products)

Synonyms: hHUS1 antibody, mHus1 antibody, XHus1 antibody, hus1 antibody, HUS1 checkpoint clamp component antibody, checkpoint clamp complex protein Hus1 antibody, HUS1 checkpoint clamp component L homeolog antibody, HUS1 antibody, Hus1 antibody, hus1 antibody, hus1.L antibody

Background:

Description: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds, endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths, and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase.

Gene: HUS1

Molecular Weight: 31kDa

Gene ID: 3364

UniProt: O60921