ITGA5 antibody (Internal Region)

Catalog No. ABIN6262610

This Rabbit Polyclonal antibody specifically detects ITGA5 in WB, IHC, ELISA, ICC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ITGA5 antibody (Internal Region) (ABIN6262610)

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITGA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-ITGA5 Antibody

Binding Specificity: Internal Region

Specificity: Integrin alpha 5 Antibody detects endogenous levels of total Integrin alpha 5.

Predicted Reactivity: Horse,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Integrin alpha 5, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ITGA5

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Alternative Name: ITGA5

Background:

Description: Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed:29030430).

Gene: ITGA5

Molecular Weight: 114 kDa

Gene ID: 3678

UniProt: P08648

Pathways: Integrin Complex