Integrin beta 4 antibody (C-Term)

Catalog No. ABIN6262617

The Rabbit Polyclonal anti-Integrin beta 4 antibody has been validated for WB, IHC, ELISA, IF and ICC. It is suitable to detect Integrin beta 4 in samples from Human, Mouse and Rat.

Quick Overview for Integrin beta 4 antibody (C-Term) (ABIN6262617)

Target: Integrin beta 4 (ITGB4)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Integrin beta 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Integrin beta 4 Antibody

Binding Specificity: C-Term

Specificity: Integrin beta 4 Antibody detects endogenous levels of total Integrin beta 4.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Integrin beta 4, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for Integrin beta 4

Target: Integrin beta 4 (ITGB4)

Alternative Name: ITGB4

Background:

Description: Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed:28873464).

Gene: ITGB4

Molecular Weight: 202 kDa

Gene ID: 3691

UniProt: P16144

Pathways: Integrin Complex